CD45RO (CD45R, CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, PTPRC, Receptor-type Tyrosine-protein Phosphatase C Polypeptide, T200, T200 Glycoprotein)

The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive in?ltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas. NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes. Low MW (Mr 180kD) isoform of the leukocyte common antigen (LCA) is a part of a family of protein tyrosine phosphatases. When activated with phytohemagglutinin (PHA), naive T-cells lose the CD45RA antigen and begin to express the CD45RO antigen. CD45RO may be a marker for memory T-cells.\n\nApplications: \nSuitable for use in Indirect Immunofluorescence, Flow Cytometry, Fluorescent Microscopy, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: Formalin-fixed paraffin embedded tissue, reacts with normal T-lymphocytes, most T-cell related tumors, true histocytic lymphomas and granulocytic sarcomas but not with B-cell related tumors.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2400-70G

Size

1ml

Applications

FC, ICC, IF, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 8% (v/v) FBS, 0.09% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG2a

References

1. Smith, S.H., et al., 1986, Immunology 58, 63. 2. Norton, A.J., et al., 1986, J. Clin. Pathol. 39, 399.

Additional Info

Recognizes human CD45RO expressed on human T-lymphocytes (40% of CD4 and 20% of CD8 positive T-lymphocytes), most thymocytes and the majority of T-cell malignancies. Also reacts with most human granulocytes and monocytes but hardly reacts with B-lymphocytes, NK-cells or Thrombocytes.

Alternative Names

EC=3.1.3.48