CD45RO (Leukocyte Common Antigen, LCA)

The CD45RA and CD45RO isoforms of the leukocyte common antigen identify functionally distinct naive and memory T cell subsets. In vitro, CD45RA+ / CD45RO - peripheral blood lymphocytes (PBL) can be converted to CD45RA- / CD45RO + phenotype upon activation in the presence of IL-2. Both CD3+ and CD3-/CD56+ lymphocyte subsets can be converted to CD45RO + lymphocytes. Expression of CD45RO was observed only in response to IL-2 and was not observed during long-term culture in IL-4, IL-6, or IL-7. This antibody recognizes an 180kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. Shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Though this antibody is useful to identify T-cell lymphomas and leukemia, rare staining with B cell lymphomas reported.\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 0.5-2ug/10e6 cells\nWestern Blot: 2-5ug/ml\nImmunohistochemistry(Paraffin): 1:250-1:500. Not required, staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20min followed by cooling at RT for 20 min. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

138519

Size

20ug

Applications

FC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a,k

References

1. Streuli M, Morimoto C, Schrieber M, Schlossman S, Saito H. J Immunol . 141:3910-3914(1988). 2. Tonks N, Charbonneau H, Diltz C, Fischer E and Walsh K. Biochem. 27:8695-8701(1988). 3. Ledbetter JA, Tonks NA, Fischer EH, Clark EA. Proc Natl Acad Sci USA 85:8628-8632(1988). 4. Knapp W, et al. Eds. Leucocyte Typing IV. Oxford University Press. New York (1989). 5. Thomas M. Annu. Rev. Immunol. 7:339 (1989). 6. Picker LJ, Treer JR, Ferguson-Darnell B, Collins PA, Buck D, Terstappen L. J Immunol . 150:1105-1121(1993). 7. Trowbridge I, et al.

Additional Info

Recognizes human CD45RO.