CD45RO (Leukocyte Common Antigen, LCA, Ly-5 T200)

CD45RO (an isoform of leukocyte common antigen) is expressed on most thymocytes, monocytes, macrophages and granulocytes. It is not expressed on normal B lymphocytes. Intrathymic T cell maturation progresses from CD45RA-/CD45RO+ to CD45RA+/CD45RO-; while peripheral T cell maturation progresses from CD45RA+/CD45RO- to CD45RA-/CD45RO+.\n\nApplications: \nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Formalin/paraffin): 1:200-1:400 for 20 minutes at RT.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nTonsil or lymph node\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2400-70

Size

500ul

Applications

FC, IHC

Hosts

Mouse

Reactivities

Hum, NHP

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2400-70X.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

1. Leukocyte Typing III, p225-227. 2. Smith S H, et. al. (1986) Immunology, 58:63-70. 3. Akbar A. N, et. al. (1988) J Immunol, 140:2171-2178. 4. Nature, 360:264-265. 5. Mori N, et al. Pathol Int, 1994, 44(2):164-9. 6. Tay YK, et al. British Journal of Dermatology, 1994, 130(6):773-6. 7. Wang HH, et al. Modern Pathology, 1994, 7(1):55-8. 8. Albrecht S et al. J of Pathol, 1993, 169:297-302. 9. Alecu M, et al. Romanian Journal of Internal Medicine, 1993, 31(3):207-12. 10. Arai E, et al. Journal of Cutaneous Pathology, 1993, 20(2):115-20. 11. Banner BF, et al. Modern Pathology, 1993, 6(3):295-301. 12. Ben-Ezra JM; Stroup RM. Transplantation, 1993, 56(1):162-5. 13. Bluth RF et al. American Journal of Clinical Pathology, 1993, 99(2):150-6. 14. Font RL, et al. Ophthalmology, 1993, 100(6):966-70. 15. Furihata M, et al. Tohoku Journal of Experimental Medicine, 1993, 169(3):187-95. 16. Hakimian D, et al. Blood, 1993, 82(6):1798-802. 17. Hayden DW, et al. Veterinary Pathology, 1993, 30(3):256-64. 18. Helm KF, et al. Journal of the American Academy of Dermatology, 1993, 29:166-70. 19. Heufelder AE; Bahn RS. Clinical and Experimental Immunology, 1993, 91(3):381-9. 20. Kanavaros P, et al. Pathology, Research and Practice, 1993, 189(1):93-8; discussion 98-101. 21. Kon S, et al. Pathology, Research and Practice, 1993, 189(10):1137-44. 22. Kurtin PJ; Roche PC. American Journal of Surgical Pathology, 1993, 17(9):898-904. 23. Liu G, et al. Human Pathology, 1993, 24(2):182-8. 24. McCarthy JM, et al. Modern Pathology, 1993, 6(5):581-7. 25. Mshana RN, et al. Parasite Immunology, 1993, 15:35-45. 26. Takahashi H, et al. Pathology, Research and Practice, 1993, 189:300-11. 27. Tallini G, et al. Journal of Clinical Gastroenterology, 1993, 17(1):57-66. 28. Welch ML, et al. Military Medical Consortium for the Advancement of Retroviral Research. Journal of the American Academy of Dermatology, 1993, 29(2 Pt 1):242-8. 29. Chadburn A, et al. Human Pathology, 1992, 23(8):940-7. 30. Clayton F, et al. Gastroenterology, 1992, 103(3):919-33. 31. Iwafuchi M, et al. Acta Pathologica Japonica, 1992, 42(5):376-81. 32. Jakic-Razumovic J, et al. Bone Marrow Transplantation, 1992, 10(1):27-31. 33. Lopez-Gil F, et al. Journal of the American Academy of Dermatology, 1992, 26(1):101-4. 34. Mangano MM, et al. Modern Pathology, 1992, 5(2):191-6. 35. Milei J, et al. Cardiology,1992,80:424-37. 36. Ohara Y, et al. Archives of Virology, 1992, 124:31-43. 37. Ratnam KV, et al. Journal of Cutaneous Pathology, 1992, 19(3):193-200. 38. Smith KJ, et al. Military Medical Consortium for Applied Retroviral Research (MMCARR). Archives of Dermatology, 1992, 128(2):193-200. 39. Sumiyoshi Y, et al. Virchows Archiv. B. Cell Pathology, 1992, 62(4):263-9. 40. Suster S. Cancer, 1992, 69(12):2910-6. 41. Travis WD, et al. Human Pathology, 1992, 23:529-41. 42. van der Wal AC, et al. European Journal of Cardio-Thoracic Surgery, 1992, 6(9):469-73; discussion 474. 43. Werner M, et al. Pathology, Research and Practice, 1992, 188(6):707-13. 44. Aozasa K, et al. Journal of Surgical Oncology, 1991, 47(4):215-20. 45. Arbustini E, et al. American Journal of Pathology, 1991, 139:709-15. 46. Berti E, et al. American Journal of Clinical Pathology, 1991, 95(2):188-93. 47. Broide DH; Firestein GS. Journal of Clinical Investigation, 1991, 88(3):1048-53. 48. Caldwell CW, et al. Clinical Immunology and Immunopathology, 1991, 58(3):377-84. 49. Campo E, et al. American Journal of Clinical Pathology, 1991, 96(2):184-90. 50. Cillari E, et al. Journal of Immunology, 1991, 146(3):1026-30. 51. Della Croce DR, et al. Hematological Oncology, 1991, 9:103-14. 52. DePalma L, et al. Transfusion, 1991, 31(8):737-42. 53. Gonzalez CL, et al. American Journal of Clinical Pathology, 1991, 96(1):81-9. 54. Hasui K. Acta Pathologica Japonica, 1991, 41(5):350-62. 55. LaSalle JM; Hafler DA. Cellular Immunology, 1991, 138(1):197-206. 56. Min KW, et al. American Journal of Clinical Pathology, 1991, 96(2):219-27. 57. Ohshima K, et al. Cancer, 1991, 67(6):1622-8. 58. Pelstring RJ, et al. Human Pathology, 1991, 22(3):237-41. 59. Regezi JA, et al. Oral Surgery, Oral Medicine, Oral Pathology, 1991, 72(6):702-8. 60. Shumack S, et al. American Journal of Dermatopathology, 1991, 13(1):26-31. 61. Skopouli FN; Fox PC; Journal of Rheumatology, 1991, 18(2):210-4. 62. Takeshita M, et al. Pathology, Research and Practice, 1991, 187(7):848-55. 63. Tateyama H, et al. Cancer, 1991, 67(8):2131-6. 64. Taubenberger JK, et al. Archives of Pathology and Laboratory Medicine, 1991, 115:338-42. 65. Toyota N, et al. Journal of Dermatology, 1991, 18(10):586-91. 66. Zukerberg LR, et al. American Journal of Surgical Pathology, 1991, 15(6):569-76. 67. Alpers CE, et al. Modern Pathology, 1990, 3(2):198-203.

Additional Info

Recognizes human CD45RO. Reacts with mature activated T-cells, most thymocytes and a sub-population of resting T-cells within both CD4 and CD8 subsets. Shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Rarely stains B cell lymphomas. Species Crossreactivity: Rhesus monkey and chimpanzee. Does not react with rat.