CD45RO (Leukocyte Common Antigen, LCA, Ly-5 T200)

Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. The CD45RO isoform contains none of the alternatively spliced exons and is present on most thymocytes, a subpopulation of resting (memory) T cells within both CD4 and CD8 subsets as well as on monocytes and granulocytes. CD45RO plays an essential role in lymphocyte activation.\n\nApplications: \nSuitable for use in Flow Cytometry and Immunohistochemistry (paraffin). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2400-70A

Size

100ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.5mg/ml gentamicin sulfate.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. S.H. Smith, et al, (1986) Immunology 58: 63-70. \n2. M. Streulo, et al, (1988) J Immunol 141: 3910-3914. \n3. M. Merkenschlager & P.C.L. Beverly, (1989) Intl Immunol 1: 450-459. I.S. \n4. Trowbridge & M.L. Thomas, (1994) Annu Rev Immunol 12: 85-116. \n5. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) \n6. p. 386-399. D. Leitenberg, et al, (1996) J Exp Med 183: 249-259.\n

Additional Info

Recognizes the CD45RO isoform of about 180kD.