CD52 (CD52 Antigen, CD52 Molecule, CDw52, Cambridge Pathology 1 Antigen, CAMPATH 1, CAMPATH-1 Antigen, Epididymal Secretory Protein E5, HE5) (PE)

The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat; 10ul labels 10e6 cells in 100ul\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C. Reconstitute by adding sterile buffer or ddH2O. Aliquot and store at 4 degrees C. DO NOT FREEZE! Reconstituted product is stable for 12 months as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2407-50J2

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b

References

1. Hale, G and Rebello, P. (1997) CD52 workshop panel report. In Leucocyte Typing VI: White Cell Differentiation Antigens. Edited by Kishimoto, T. et al. Oxford University Press pp 514 - 517\n2. Hale, G. (2001) Cd52 (Campath1). J. Biol. Regul. Homeost. Agents. 15 (4): 386 - 391.

Additional Info

Recognizes human CD52.