CD58 (Ag3, FLJ23181, FLJ43722, LFA3, LFA-3, Lymphocyte function-associated antigen 3, Surface glycoprotein LFA-3) (FITC)

Human CD58 is a cell surface glycoprotein that functions as an adhesion molocule and mediates costimulatory activity through its ligand CD2, which is expressed on T and natural killer cells (1,2). CD58 has wide cellular distribution on both hemopoietic and non-hemopoietic cells with strong expression on macrophages and memory T cells. \n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 5x10e5 cultured Nalm-6 cells were washed after which they were incubated 45 min on ice with 80ul of anti-CD58 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.46 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

C2412-05J

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as liquid in 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150mM sodium chloride, 0.5 mg/ml gentamicin sulfate. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 414-415, 2. A.R. Arulanandam, et al, (1994) J Exp Med 180:1861-1871.

Additional Info

Recognizes the human CD58 glycoprotein of about 60-70kD. Inhibits HLA-DR mediated T cell cytotoxicity.