CD90 (THY1.1)

The Thy-1 antigen is found on a variety of cell types including thymocytes, neuronal cells, T-Lymphocytes (mouse), stem cells and immature B cells (rat) and connective tissue.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested. \n\nRecommended Dilution: \nFlow Cytometry: Use 10ul to label 10e6 cells in 100ul.\nOptimal dilution determined by the researcher. \n\nHybridoma: \nSpleen cells of immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2441-04B

Size

50ug

Applications

FC

Hosts

Mouse

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.\nConjugated to Fluorescein Isothiocyanate Isomer 1 (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Mason, D.W. and Williams, A.F. (1980). Biochem.J. 187: 1-20. 2. Campbell, D.G. et al. (1981). Biochem. J. 195: 15-30. 3. Bukovsk

Additional Info

Recognizes mouse and rat CD90, also known as Thy1.1. Thy1.1 is a monomorphic determinant in rat but polymorphic in mice. Thus clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c.\n