Home  >  Products  >  Centaurin beta 2 (ACAP2, Ankyrin repeat, ARF GAP with coiled coil ANK repeat and PH domains 2, CENTB2, Cnt b2, KIAA0041, Pleckstrin homology domains 2)
Centaurin beta 2 (ACAP2, Ankyrin repeat, ARF GAP with coiled coil ANK repeat and PH domains 2, CENTB2, Cnt b2, KIAA0041, Pleckstrin homology domains 2)

Centaurin beta 2 (ACAP2, Ankyrin repeat, ARF GAP with coiled coil ANK repeat and PH domains 2, CENTB2, Cnt b2, KIAA0041, Pleckstrin homology domains 2)

Cat no: C2617-02D

Supplier: United States Biological
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Centaurin beta 1 and Centaurin beta 2 are recruited to platelet-derived growth factor-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. In vitro, Centaurin beta 1 and Centaurin beta 2 preferred ARF6 as substrate rather than ARF1 or ARF5, and the GAP activity of both Centaurins was dependent upon phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). The isolated PH domain of mouse Centaurin beta 2 exhibits moderate affinity for PtdIns(3,5)P2, but it does not bind any other phosphoinositides tested, including PtdIns(4,5)P2. Mutation of a highly conserved arginine in both Centaurins result in lack of ARF-GAP activity. Overexpression in HeLa cells of either Centaurin blocks the formation of ARF6-dependent protrusions. Both Centaurins are also recruited to peripheral, tubular membranes, where activation of ARF6 occurs and allows membrane recycling back to the plasma membrane. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32000 Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2617-02D
Reactivities: Human, Mouse
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Jackson TR, Brown FD, Nie Z, Miura K, Foroni L, Sun J, Hsu VW, Donaldson JG, Randazzo PA. ACAPs are arf6 GTPase-activating proteins that function in the cell periphery. J Cell Biol. 2000 Oct 30;151(3):627-38.
Additional info: Recognizes human ACAP2 at ~90kD in human T cell line MOLT4 lysates (calculated MW of 88.1kDa according to NP_036419). Species Crossreactivity: Mouse.

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