CENTB1 (Arf-GAP with Coiled-coil, ANK Repeat and PH Domain-containing Protein 1, Centaurin-beta-1, Cnt-b1, ACAP1, KIAA0050)

ACAP1, also known as Centaurin-beta-1 (CENTB1), is a 740aa containing transport carrier belonging to the Centaurin family with three ANK repeats, an Arf-GAP domain, a BAR domain and a PH domain that binds to phospholipids. ACAP1, a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6), is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. It mediates CENTB1-binding to phosphatidylinositol 3,5-bisphosphate (PIP2) or phosphatidylinositol 3,4,5-trisphosphate (PIP3) containing membranes. Along with ACAP2, ACAP1 is recruited to platelet-derived growth factor (PDGF) induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, while over expression inhibited ruffle formation. It is widely expressed in most tissues with high expression in lung and spleen and low expression in heart, kidney, liver and pancreas.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nK562 cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2617-02E1

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Dai, J. et al. DNA Res. 1:223-229 (1994). 2. Jackson, TR. et al. J. Cell Biol. 151:627-638 (2000). 3. Nomura, N. et al. Dev. Cell. 7:771-776 (2004).

Additional Info

Recognizes human CENTB1. Species sequence homology: mouse and bovine.