CES2 (Carboxylesterase 2; also CE-2) is a glycosylated, monomeric member of the type-B carboxyesterease/lipase family of enzymes. Although it runs at 59-62kD in SDS-PAGE, Gel filtration assigns an 80kD MW to it. CES2 is an ER luminal enzyme that catalyzes the conversion of carboxlic ester to alcohol. It is found in small intestine and liver, and likely participates in the detoxification of drugs. Mature human CES2 is 533 amino acids (aa) in length (aa27-559). It contains a catalytic site composed of Ser228Glu345His457, and an ER-retention motif overaa 556-559. Individually or in combination, splice variants may show an alternate start site 64aa upstream of the standard site, a Val substitution foraa 458-474, or a nine aa insertion after Met1. Over the length of the precursor (aa1-559) human CES2 shares 72% aa identity with mouse CES2.
Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 0.1-0.2ug/ml
ELISA (detection): 0.1-0.4ug/ml
Immunohistochemistry: 3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile TBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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