CFP10 (mtsA10, 10kD culture filtrate antigen cfp10, Culture filtrate protein 10, ESAT6 like protein esxB, esxB, lhp, Secreted antigenic protein MTSA 10 )

CFP10 (10kD culture filtrate protein) is a low molecular weight protein isolated from Mycobacterium tuberculosis culture filtrates. CFP10 and the 6kD early secretory antigen of T cells (ESAT6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Bacille Calmette Guerin (BCG). CFP10 is one of a small number of secreted M. tuberculosis proteins now identified, that appear to play key roles in tuberculosis pathogenesis and in the stimulation of immunity. This antibody was raised by a genetic immunization technique. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.2-1ug/ml under reducing conditions.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C3199-01B

Size

100ug

Applications

WB

Hosts

Rabbit

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography. >95% by SDS-PAGE.

Isotype

IgG

Additional Info

Recognizes CFP10 (LHP), a 12,3kD antigen encoded by gene Rv3874 of Mycobacterium tuberculosis. The antibody recognizes CFP10 of Mycobacterium tuberculosis H37Rv and Mycobacterium bovis; it reacts also with recombinant antigen produced in Escherichia coli.

Alternative Names

mtsA10, 10kD culture filtrate antigen cfp10, Culture filtrate protein 10, ESAT 6 like protein esxB, esxB, lhp, \nSecreted antigenic protein MTSA 10