Chaperonin 10 (Cpn10, Heat Shock Protein 10, HSP10)

Chaperonin 10 (Cpn 10 or Hsp10), is the ~10kD mammalian equivalent to GroES of E. coli. Chaperonins play fundamental roles in the folding, assembly, and translocation of other proteins. Chaperonin function in vivo requires two distinct family members, cpn60 and cpn10, both of which are also present in chloroplasts and mitochondria. The best studied cpn60 and cpn10, are the GroEL and GroES proteins of E. coli, respectively. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly processes. Chaperonin 10 has been located in human platelets but more importantly it may also be present in human maternal serum. It has been reported that human cpn10 is identical with early pregnancy factor (EPF), which is involved in control over cell growth and development. This identification suggests that cpn10 may act like a hormone in stressful situations such as pregnancy.\n \nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot (Colorimetric): 1:1000 \nWestern Blot (ECL): 1:1000-1:2500 \nImmunoprecipitation: 1:50 \nOptimal dilutions to be determined by the researcher. \n \nPositive Controls:\nHeat Shocked HeLa Cell Lysate, \nC3450 Chaperonin 10, Recombinant, Human (cpn10, Heat Shock Protein 10, Hsp10)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C3470

Size

50ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, GP, Prc, Rabbit, Sheep, Xen/Amph

Form

Supplied as a liquid, in PBS, pH 7.2, 0.09% azide, 50% glycerol

P Type

Pab

Purity

Purified by Protein A affinity chromatography

Isotype

IgG

References

1. Hemmingsen, S.M., et al., Nature 333: 330-334 (1988). 2. Gething, M.-J. & Sambrook, J., Nature 355: 33-45 (1992). 3. Gatenby, A.A. & Viitanen, P.V., Annu. Rev. Plant Physiol. Plant Mol. Biol. 45: 469-491 (1994). 4. Morton, H., et al., Nature (London) 249: 459-460 (1974). 5. Cavanagh, A.C. & Morton, H., Eur. J. Biochem. 222: 551-560 (1994). 6. Samali, A., et al., EMBO J. 18: 2040-2048 (1999). 7. Ravagnan, L., et al., Nat. Cell Biol. 3(9): 839-843 (2001). 8. Ruepp, S.U., et al., Toxicol. Sci. 65(1): 135-150 (2002). 9. Schafler, A.E., et al., Ann. Thorac. Surg. 74(3): 767-770 (2002). 10. Wilson-Fritch, L., et al., Mol. Cell Biol. 23(3): 1085-1094 (2003).

Additional Info

Recognizes a protein, corresponding to the apparent molecular mass of rat Cpn10 on SDS-PAGE immunoblot. at ~10kD. Species Crossreactivity: Human, mouse, bovine, canine, guinea pig, porcine, rabbit, sheep and Xenopus.