Chaperonin 10 (Cpn10, Heat Shock Protein 10, HSP10) (Chlamydia)

Homologs of E. coli GroES make up a family of small heat shock proteins with an approximate molecular mass of 10kD (Hsp10s). Hsp10 acts as a cochaperone and interacts with members of the Hsp60 family (GroEL in E. coli) to promote proper folding of polypeptides. In chlamydia, cpn10 is thought to interact with cHsp60, a reported immunopathological antigen (2), identifying cpn10 as a potential contributor to an immunological response (1). \n \nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and ELISA. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 0.25ug/ml \nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C3470A

Size

25ug

Applications

ELISA, ICC, IP, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, 0.1mM PMSF, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. LaVerda, D. and Byrne, G.I. (1997) J. Clin. Micro. 35: 1209-1215. \n2. Morrison, R.P., Belland, R.J., Lyng, K., and Caldwell, H.D. (1989) J. Exp. Med. 170: \n1271-1283.

Additional Info

Detects a 15kD protein, corresponding to the apparent molecular mass of cpn10 on SDS-PAGE immunoblots, in C. trachomatis infected cells. This antibody will not react with the human or mouse cpn10 homolog nor with E. coli GroES.

Alternative Names