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CHD3 (Chromodomain Helicase DNA Binding Protein 3, CHD-3, ATP-dependent Helicase CHD3, hZFH, Mi-2a, Mi-2 alpha, Mi-2 Autoantigen 240kD Protein, ZFH, Zinc-finger Helicase (Snf2-like)) (Biotin)

Cat no: C3875-50D-Biotin

CHD3 (Chromodomain Helicase DNA Binding Protein 3, CHD-3, ATP-dependent Helicase CHD3, hZFH, Mi-2a, Mi-2 alpha, Mi-2 Autoantigen 240kD Protein, ZFH, Zinc-finger Helicase (Snf2-like)) (Biotin)

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 0.1-10ug/ml\nELISA: 1ug/ml-3ng/ml\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSp2/0 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C3875-50D-Biotin

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

Additional Info

Recognizes human CHD3.

Alternative Names

2600010P09Rik, AF020312, MGC40857, Prp9-1

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