Cherry (mCherry, DsRed, Discosoma)

mCherry is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kD protein isolated originally from the jellyfish Aequoria victoria. GFP was the basis of the 2008 Nobel Prize in Chemistry, awarded to Osamu Shimomura, Martin Chalfie and Roger Tsien, specifically "for the discovery and development of the green fluorescent protein, GFP". On expression from the GFP gene, GFP protein will fold correctly and fluoresce strongly, the development of fluoresence requires no cofactors except molecular oxygen. It can therefore be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell under aerobic conditions (1). As a result DNA encoding GFP can be fused to DNA encoding other proteins as a means to visualize the resulting fusion protein in live cells or animals. Engineered forms of GFP and relatives have been developed to monitor Calcium levels, protease activation and in a variety of other processes in real time. A whole range of GFP derivatives with different spectral properties have been developed, largely in the Tsien lab. The mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kD protein similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties (2-4). The resulting monomeric protein is useful for applications such as F

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SPECIFICATIONS

Catalog Number

031353

Size

100ul

Applications

IF, WB

Hosts

Mouse

Form

Supplied as a liquid in 10mM sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC Green fluorescent protein as a marker for gene expression. Science. 263:802-5 (1994).\n\n2. Baird GS, Zacharias DA, Tsien RY. Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. Proc Natl Acad Sci U S A. 97:11984-9 (2000).\n\n3. Gross LA, Baird GS, Hoffman RC, Baldridge KK, Tsien RY. The structure of the chromophore within DsRed, a red fluorescent protein from coral. Proc Natl Acad Sci U S A. 97:11990-5 (2000).\n\n4. Heikal AA, Hess ST, Baird GS, Tsien RY, Webb WW. Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: coral red (dsRed) and yellow (Citrine). Proc Natl Acad Sci U S A. 97:11996-2001 (2000).\n\n5. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22:1567-1572 (2004).

Additional Info

Recognizes mCherry.