Mouse Chitinase 3-like 3 (CHI3L3), also known as ECF-L (eosinophil chemotactic factor-lymphocyte) or Ym1, is a secreted ~45kD glycoprotein that is a member of the glycosyl hydrolase family 18 (chitinase-like) protein family. Mouse CHI3L3 has no ortholog in humans, but shares 80% and 90% aa sequence identity with rat CHI3L3 and mouse CHI3L4 (also known as Ym2), respectively. Mouse CHI3L3 and CHI3L4 share partial overlap in cell type and tissue mRNA expression. CHI3L3 is primarily secreted by alveolar and peritoneal macrophages during inflammation, for example, due to nematode infection and airway hyper-responsiveness. It is considered a marker for alternatively activated macrophages, and is also expressed in bone marrow myeloid precursors, activated microglia, bone marrow-derived immature mast cells, connective tissue-type mast cells, macrophages in erythroblastic islands, neutrophil granules in bone marrow, peritoneum and spleen red pulp, and IL-4-stimulated B cells and dendritic cells (DC).
Applications:
Suitable for use in ELISA and Western Blot Other applications not tested.
Recommended Dilutions:
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant mouse ECF-L is ~3ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse ECF-L is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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