Chitotriosidase (Chitinase 1, CHIT-1)

Human CHIT-1 (chitotriosidase-1; also chitinase-1) is a 50kD, secreted glycoprotein that belongs to glycosylhydrolase family #18. It is synthesized by macrophages, and may serve to degrade chitin (poly-N-acetylglucosamine)-containing pathogens such as fungi and nematodes. Mature CHIT-1 is 435 amino acids (aa) in length, and contains a catalytic domain (aa #22-372) followed by a chitin-binding region (aa #373-466). Very high levels of CHIT1 are found in the plasma of patients with Gaucher's disease type I. Mature human CHIT-1 is 79% aa identical to mouse CHIT-1.\nApplications: \nSuitable for use in ELISA, Immunoprecipitation and Neutralization. Other applications not tested.\n\nRecommended Dilution:\nELISA (direct): 0.5-1.0ug/ml. Detection limit for rhCHIT1 is approximately 2ng/well.\nImmunoprecipitation: 25ug/ml to immunoprecipitate rhCHIT1 from conditioned cell culture medium. \nNeutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 min. The enzyme was then assayed with 4-MU-beta-D-N,N',N''-triacetylchitotriose. 50% of the enzymatic activity was inhibited by the antibody at approximately 56ng/mL (IC 50) under conditions in which the enzyme was present at 20ng/mL and the substrate concentration was 0.01mM. IC 50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nFusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human CHIT1.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C4164-50

Size

100ug

Applications

ELISA, IP

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human CHIT1 in direct ELISAs and Western Blots. In these formats, this antibody shows no crossreactivity with rhCHI-3L1. Species sequence homology: mouse: 79%.