Chk1, NT (CHEK1, Cell Cycle Checkpoint Kinase)

Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4 ). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blotting: 1:1,000\nImmunocytochemistry: 1:50\nImmunoprecipitation: 1:80\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\n\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

C4200-05B

Size

100ul

Applications

ICC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Martinho, R.G., et al. Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses. EMBO J. 17: 7239-7249 (1998). 2. Zhao, H. and H. Piwnica-Worms. ATR-mediated checkpoint pathways regulate phosphorylation and activation of human Chk1. Mol. Cell. Biol. 21: 4129-4139 (2001). 3. Zeng, Y., et al. Replication checkpoint requires phosphorylation of the phosphatase Cdc25 by Cds1 or Chk1. Nature 395: 507-510 (1998). 4. Sanchez, Y., et al. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science 277: 1497-501 (1997).

Additional Info

Recognizes human Chk1. Species crossreactivity: mouse and rat.