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CHKB (choline kinase beta, CHETK, CHKL, choline kinase-like, choline/ethanolamine kinase)

CHKB (choline kinase beta, CHETK, CHKL, choline kinase-like, choline/ethanolamine kinase)

Cat no: C5062-70

Supplier: United States Biological
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The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family. Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C5062-70
Reactivities: Human
Hosts: Goat
Applications: ELISA
Size: 100ug
Form: Supplied as a liquid in Tris, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Purity: Purified by affinity chromatography.
References: 1. Aoyama C, Liao H, Ishidate K. Structure and function of choline kinase isoforms in mammalian cells. Prog Lipid Res. 2004 May;43(3):266-81. PMID: 15003397
Additional info: Recognizes human CHKB. Species sequence homology: canine.

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