CHMP2B (Charged Multivesicular Body Protein 2b, CHMP2.5, Chromatin-modifying Protein 2b, CHMP2b, Vacuolar Protein Sorting-associated Protein 2-2, Vps2-2, hVps2-2, CGI-84)

CHMP2B (CHarged Multivescular body Protein 2B? also Chromatin Modifying Protein 2B and Vps22) is a 35kD member of the SNF family of proteins. It is a cytosolic molecule that interacts with VPS4 and undergoes polymerization to form tubules that project plasma membrane outward, generating an environment conducive to membrane fission and budding. It also appears to participate in the formation of intraluminal vesicles associated with the lysosomal system. CHMP2B is found in striated muscle (heart and skeletal), and particularly in neurons of the CNS. Human CHMP2B is 213aa in length. It contains one coiled-coil region (aa25-55), an MIT (microtubule-interacting and trafficking) domain (aa201-211) and a utilized phosphorylation site at Ser199. There is one alternative splice form that shows a deletion of aa2-42. Full length human CHMP2B shares 99% aa identity with mouse CHMP2B, but only 33% aa identity with human CHMP2A.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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