Cholera Toxin, beta, Vibrio cholerae

Cholera toxin is an oligomeric protein of MW 84kD and consists of a single A subunit surrounded by five B subunits. It is a potent activator of adenylate cyclase and is the pathogenic agent responsible for the symptoms of cholera. The B subunit (choleragenoid) is responsible for the binding of the holotoxin to G M1 ganglioside receptors on mammalian cell surfaces and facilitates entrance of the A subunit into the cell. The A subunit bears the ADP-ribosyl-transferase activity, which deregulates the G sprotein causing activation of adenylate cyclase. Due to the ubiquitous occurrence of the GM1 ganglioside receptor on eukaryotic cell membranes, cholera toxin activates adenylate cyclase in a wide variety of systems.\nCholera toxin has become a powerful research tool not only in microbiology, but in the fields of physiology, cell biology and biochemistry, as well. Because of the effect on adenylate cyclase, cholera toxin and its purified A subunit are frequently used for the study of signal transduction mechanisms. In addition, cholera toxin acts as an adjuvant through the stimulation of B-lymphocytes. The cholera toxin B subunit alone is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.\n\nApplications:\nSuitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry: Frozen sections\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C4275-35J

Size

200ug

Applications

ELISA, IHC

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography

Isotype

IgG1

Additional Info

Recognizes the labile entertoxin normally associated with Vibrio cholerae and reacts with an epitope on the beta chain. No crossreactivity with various E. coli serotypes.