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Cholesterol Acyltransferase 1 (Acyl Coenzyme A Cholesterol Acyltransferase 1, ACAT1, Sterol O-Acyltransferase 1)

Cholesterol Acyltransferase 1 (Acyl Coenzyme A Cholesterol Acyltransferase 1, ACAT1, Sterol O-Acyltransferase 1)

Cat no: C5045-33C

Supplier: United States Biological
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ACAT-1 is a ~50kD protein that catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys. Positive Control: CHO cell lysate Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:400. This antibody recognizes a non-specific band at ~ 30kD, in addition to the specific ACAT1 protein band at 46kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C5045-33C
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: As reported
P type: Pab
Purity: Serum
Alternative names: ACAT1, ACAT, Acetoacetyl CoA thiolase, Acetoacetyl Coenzyme A thiolase, Acetyl Coenzyme A acetyltransferase 1, MAT, T2, THIL
References: 1. Chang, CCY. et al. Regulation and immunolocalization of Acyl-Coenzyme A: Cholesterol Acyltransferase in Mammalian Cells as Studied with Specific Antibodies. <i>JBC</i> <b>270:</b> 29532(1995) 2. Song, J., Kost, C. K., Jr., and Martin, D. S. (2006) Androgens augment renal vascular responses to ANG II in New Zealand genetically hypertensive rats, 290, R1608-1615.
Additional info: This antibody is specific for ACAT1. Species Crossreactivity: Human, hamster and mouse. Other species have not been tested.

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