Cholesterol Acyltransferase 1 (Sterol O-acyltransferase 1, Acyl-coenzyme A:cholesterol Acyltransferase 1, ACAT-1, SOAT1, ACACT, ACACT1, ACAT, ACAT1, SOAT, STAT)

Cholesterol acyltransferase 1 is mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA and plays a significant role in ketone body metabolism. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. Recent studies suggests that ACAT1 may be a therapeutic target for treating certain forms of Alzeheimer's disease.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.05ug/ml\nImmunohistochemistry: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nCaco-2 cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029444

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Maurer, G.D., et al. (2011). BMC Cancer. 11(1):315. 2. Bryleva, E.Y., et al. (2010). Proc Natl Acad Sci USA. 107(7):3081-3086. 3. Catanzano, F., et al. (2010). J Inherit Metab Dis. 423-431. 4. Fukao, T., et al. (1992). J Clin Invest. 89(2): 474-479.

Additional Info

Recognizes human Cholesterol Acyltransferase 1. Species sequence homology: Rhesus macaque, chimpanzee, rat, mouse (93%).