Acyl-Coenzyme A is cholesterol acyltransferase-2 (ACAT-2) which catalyzes the formation of cholesterol esters from cholesterol and long chain fatty acyl-coenzyme A. It is associated with lipoprotein particle secretion and thus plays an important role in the metabolism of triglyceride-rich lipoproteins like ApoB100.1,2,3 In humans, ACAT-2 is primarily expressed in the small intestine, whereas in other mammals, the enzyme is expressed in the liver and the small intestine.4,2 Human ACAT-2 has 522 amino acid with an estimated molecular weight of 60 kD.5 Detects ACAT-2 at about 60 kD in murine liver, intestine, and fibroblasts.6
Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution:
Immunocytochemistry: 1:500, 1us/ml
Immunohistochemistry: 1:500, 1ug/ml
Western Blot: 1:250, 2ug/ml for 1 hour at RT. Overnight incubations at 4C with greater dilutions can produce optimal results.
Optimal dilution to be determined by researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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