Cholesterol Esterase (Carboxyl Ester Lipase, CEL, Lysophosphatidylcholine, Bile salt-stimulated lipase, BAL, BSDL, BSSL, Bucelipase, CEase, CELL, , lysoPC, PTL, Pancreatic triglyceride lipase, FAP, FAPP, LIPA, MODY8, Pancreatic lysophospholipase, Sterol e

Cholesterol Esterase is found primarily in pancreas and pancreatic secretions, but in other tissues as well. Bile salts, such as cholate and its conjugates, act as stabilizers of the native polymeric form of the enzyme and can protect it from proteolytic degradation in the intestine. Cholesterol esterase is an enzyme that catalyses the hydrolysis of cholesterol and some other sterol esters to liberate cholesterol plus a fatty acid anion.\n\nThe enzyme is bound to cellular membranes of excitable tissue (synaptic junction, endoplasmic reticulum, etc.). It is believed to be associated with nerve impulse conduction.\n\n\nProtein Accession Number: NP_001116606\n\nMolecular Weight:\nMonomer: 65kD (8)\nHexamer: 400kD (8) \n\nOptimal pH: \nFor esterification, 6.1-6.2\nFor hydrolysis, 6.6-7.0 (17).\n\nActive Site Residues:\nSerine (S194)\nHistidine (H435)\nAspartate (D320)\n\nActivators:\nBile Salts, Cholate, glycocholate, taurocholate (18).\n\nInhibitors:\nPMSF and p-chloromercuribenzoate (8)\nDiisopropyl fluorophosphate (Momsen and Brockman 1976)\nHg2+, Ag+, and ionic detergents\nAryl carbamates (Feaster et al. 1996)\n\nProtease: <80ug/unit\n\nActivity (u/gm dw): (same/more than)300 \n\nUnit Definition: \n1unit hydrolyzes 1umole of cholesterol ester per minute at 37C, pH 7.0.\n\nConcentration (A280): ~1.5mg/ml\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 0.1M PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5047

Size

500U

Form

Supplied as a lyophilized powder. Reconstitute with sterile 0.1M PBS to 1mg/ml.

Purity

Chromatographically purified.\nProtease: <80ug/unit

References

1. Allain, C.C., et al., Clin Chem 20, 470, 1974 2. Camulli, E., et al., Biochim Biophys Acta 1005, 177, 1989 3. Cox, D., et al., Biochem 29, 3842, 1990 4. Dipersio, L., et al., J Biol Chem 266, 4033, 1991 5. Hernandez, H., et al., J Gen Physiol 40, 447, 1957 6. Huang, Y., et al., J Lipid Res 31, 2029, 1990 7. Hui, D., et al., FEBS Lett 276, 131, 1990 8. Hyun, J., et al., Biochem Biophys Res Commun 44, 819, 1971 9. Kyger, et al., Biochem 29, 3853, 1990 10. Kyger, R., et al., Biochem Biophys Res Commun 164, 130, 1989 11. Mori, S., et al., Biotechnol Appl Biochem 16, 101, 1992 12. Nakamura, K., Iet al., Biochim Biophys Acta 963, 320-329, 1988 13. Riley, D., et al., Biochem 29, 3848, 1990 14. Rudd, E., Biochim Biophys Acta 918, 106, 1987 15. Slotte, J., et al., Biochim Biophys Acta 958, 308, 1988 16. Sohl, J., et al., Biochem Biophys Res Commun 151, 554, 1988 17. Vahouny, G.V., et al., Methods of Biochem. Anal. 16, 219, 1968 18. Vahouny, G.V., et al., Biochem Biophys Res Commun 15, 224, 1964

Alternative Names

EC=3.1.1.13; Porcine pancreas