Cholesterol Esterase (Carboxyl Ester Lipase, CEL, Lysophosphatidylcholine, Bile salt-stimulated lipase, BAL, BSDL, BSSL, Bucelipase, CEase, CELL, , lysoPC, PTL, Pancreatic triglyceride lipase, FAP, FAPP, LIPA, MODY8, Pancreatic lysophospholipase, Sterol e

Cat no: C5047

Supplier: United States Biological

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Cholesterol Esterase is found primarily in pancreas and pancreatic secretions, but in other tissues as well. Bile salts, such as cholate and its conjugates, act as stabilizers of the native polymeric form of the enzyme and can protect it from proteolytic degradation in the intestine. Cholesterol esterase is an enzyme that catalyses the hydrolysis of cholesterol and some other sterol esters to liberate cholesterol plus a fatty acid anion. The enzyme is bound to cellular membranes of excitable tissue (synaptic junction, endoplasmic reticulum, etc.). It is believed to be associated with nerve impulse conduction. Protein Accession Number: NP_001116606 Molecular Weight: Monomer: 65kD (8) Hexamer: 400kD (8) Optimal pH: For esterification, 6.1-6.2 For hydrolysis, 6.6-7.0 (17). Active Site Residues: Serine (S194) Histidine (H435) Aspartate (D320) Activators: Bile Salts, Cholate, glycocholate, taurocholate (18). Inhibitors: PMSF and p-chloromercuribenzoate (8) Diisopropyl fluorophosphate (Momsen and Brockman 1976) Hg2+, Ag+, and ionic detergents Aryl carbamates (Feaster et al. 1996) Protease: <80ug/unit Activity (u/gm dw): (same/more than)300 Unit Definition: 1unit hydrolyzes 1umole of cholesterol ester per minute at 37C, pH 7.0. Concentration (A280): ~1.5mg/ml Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 0.1M PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	C5047
Size:	500U
Form:	Supplied as a lyophilized powder. Reconstitute with sterile 0.1M PBS to 1mg/ml.
Purity:	Chromatographically purified. Protease: <80ug/unit
Alternative names:	EC=3.1.1.13; Porcine pancreas
References:	1. Allain, C.C., et al., Clin Chem 20, 470, 1974 2. Camulli, E., et al., Biochim Biophys Acta 1005, 177, 1989 3. Cox, D., et al., Biochem 29, 3842, 1990 4. Dipersio, L., et al., J Biol Chem 266, 4033, 1991 5. Hernandez, H., et al., J Gen Physiol 40, 447, 1957 6. Huang, Y., et al., J Lipid Res 31, 2029, 1990 7. Hui, D., et al., FEBS Lett 276, 131, 1990 8. Hyun, J., et al., Biochem Biophys Res Commun 44, 819, 1971 9. Kyger, et al., Biochem 29, 3853, 1990 10. Kyger, R., et al., Biochem Biophys Res Commun 164, 130, 1989 11. Mori, S., et al., Biotechnol Appl Biochem 16, 101, 1992 12. Nakamura, K., Iet al., Biochim Biophys Acta 963, 320-329, 1988 13. Riley, D., et al., Biochem 29, 3848, 1990 14. Rudd, E., Biochim Biophys Acta 918, 106, 1987 15. Slotte, J., et al., Biochim Biophys Acta 958, 308, 1988 16. Sohl, J., et al., Biochem Biophys Res Commun 151, 554, 1988 17. Vahouny, G.V., et al., Methods of Biochem. Anal. 16, 219, 1968 18. Vahouny, G.V., et al., Biochem Biophys Res Commun 15, 224, 1964

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Cholesterol Esterase (Carboxyl Ester Lipase, CEL, Lysophosphatidylcholine, Bile salt-stimulated lipase, BAL, BSDL, BSSL, Bucelipase, CEase, CELL, , lysoPC, PTL, Pancreatic triglyceride lipase, FAP, FAPP, LIPA, MODY8, Pancreatic lysophospholipase, Sterol e

Cat no: C5047

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