Choline Acetyltransferase (CAT, CHAT)

Acetylcholine (Ach) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransfrase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.\n\nApplications:\nSuitable for use in Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry (Paraffin): 1:100 staining on normal cerebral cortex. Tissue not treated with antigen retrieval. Immunoreactivity is seen as a staining pattern on the cell body, dendrites and axons of neurons.\nImmunoprecipitation: 1:200. Immunoprecipitates 68kD active ChAT from brain and placenta. Also precipitates a 27kD protein that is co-expressed with ChAT (but lacking ChAT activity) in some cholinergic neurons of the central nervous system of humans and possibly other mammals. \nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nCerebral cortex tissue, normal\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5057-05

Size

50ul

Applications

IHC, IP

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Fel, NHP

Form

Supplied as a liquid. No preservative added.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Brain Research Bulletin (1987) 18:447-455. 2. Sleep (1988) 11:1-16. 3. Neuroscience (1989) 32:685-700. 4. Brain Research (1990) 517:224-228. 5. Brain Research (1990) 532:317-322. 6. J. Neuroscience Methods (1990) 35:31-37. 7. Soc. Neuroscience Abstracts (1992) Vol 18, pg 1483, #624.2. 8. Brain Research Bulletin (1990) 25:129-137. 9. Brain Research Bulletin (1992) 29:635-642. 10. Neuroscience Letters (1992) 147:217-220. 11. Brain Research (1993) 627:49-54 12. Neurobiology of Aging (1993) 14:389-392 13. Cell (1993) 75:1273-1286. 14. Cell (1993) 75:1199-1214. 15. Brain Research (1993) 223-232. 16. Neuroscience (1993) Vol. 54 No. 4:845-857. 17. Experimental Neurology (1994) 130:230-236. 18. Neuroscience Letters (1994) 180:214-218. 19. J. Neurochemistry (1995) 65:484-491. 20. J. Comparative Neurology (1996) 364:104-112. 21. J. Comparative Neurology (1996) 364:113-120. 22. Microscopy and Microanalysis (1997) 3:53-69. 23. J. of Neuroscience (1998) 18(21):8936-8946. 24. European J. of Neuroscience (1998) 10:2026-2036. 25. Neuroscience (1998) 84:1113-1125. 26. J. Cerebral Blood Flow and Metabolism (1999) 19:1220-1228. 27. Masliah, E. et al., PNAS.USA (2001) 98:12245-12250.

Additional Info

Recognizes Cholinergic neurons in human brain and central nervous systems. Species Crossreactivity: Rat, mouse, feline, monkey and bat.