Home  >  Products  >  Choline Acetyltransferase (ChAT, Choline O-acetyltransferase, Acetyl CoA Choline O-acetyltransferase, CHOACTase, Choline Acetylase, CMS1A, CMS1A2, EC 2.3.1.6)
Choline Acetyltransferase (ChAT, Choline O-acetyltransferase, Acetyl CoA Choline O-acetyltransferase, CHOACTase, Choline Acetylase, CMS1A, CMS1A2, EC 2.3.1.6)

Choline Acetyltransferase (ChAT, Choline O-acetyltransferase, Acetyl CoA Choline O-acetyltransferase, CHOACTase, Choline Acetylase, CMS1A, CMS1A2, EC 2.3.1.6)

Cat no: C5057-02E

Supplier: United States Biological
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Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetylase, aka choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunohistochemistry: 1:200 Optimal dilutions to be determined by the researcher. Positive Control: Human fetal brain tissue lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C5057-02E
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human Choline acetylase. Species Crossreactivity: rat, mouse.

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