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Choline Acetyltransferase (CHAT, CMS1A, CMS1A2, Acetyl CoA:choline O-acetyltransferase)

Choline Acetyltransferase (CHAT, CMS1A, CMS1A2, Acetyl CoA:choline O-acetyltransferase)

Cat no: C5057-05B

Supplier: United States Biological
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Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. Belongs to the carnitine/choline acetyltransferase family. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C5057-05B
Reactivities: Human, Rat, Guinea Pig, Rabbit
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
P type: Pab
Purity: Serum
Alternative names: Chat, CHAT, CMS1A, CMS1A2, Acetyl CoA:choline O-acetyltransferase
References: Benecke et al. (1993). J. Neurochemistry. 61: 804-811
Additional info: This antiserum stains cholinergic neurons in human, rat, guinea-pig and rabbit central and peripheral nervous systems. Species Crossreactivity: This antiserum is known to react with ChAT of origin: human, rat, guinea-pig and rabbit.

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