Chondroitin 4 Sulfate

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.\n\nApplications: \nSuitable for use in ELISA, RIA, Immunohistochemistry and Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nELISA: 1:400-1:3200 \nRIA: 1:50-1:6400\nWestern Blot: 1:1000-1:4000 (ECL)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5067-01

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Sheep

Form

Supplied as a liquid.

P Type

Mab

Purity

Ascites

Isotype

IgG1,k

References

1. Ortega-Barria, E et al. (1999). A Toxoplasma Lectin-like Activity Specific for Sulfated Polysaccharides Is Involved in Host Cell Infection. J Biol Chem. 274:1267-1276. 2. Glant, T. et al. (1998). Critical roles of glycosaminoglycan side chains of cartilage proteoglycan (aggrecan) in antigen recognition and presentation. J. Immunology 160:3812- 3819. 3. Cs-Szabo, TG. et al. (1995). Large and small proteoglycans of osteoarthritic and rheumatoid articular cartilage. Arthritis Rheum 38:660-668. 4. Poole, C. et al. (1991). Chondrons from articular cartilage. (IV). Immunolocalization of proteoglycan epitopes in isolated canine tibial chondrons. J. Histochem. Cytochem. 39:1175-1187. 5. Mikecz, K. et al. (1987). Immunity to cartilage proteoglycans in BALB/c mice with progressive polyarthritis and ankylosing spondylitis induced by injection of human cartilage proteoglycan. Arthritis Rheum. 30:306-318.

Additional Info

Recognizes both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. Chondroitin 4 sulfate disaccharides successfully inhibit the binding of monoclonal antibody to proteoglycans. No reaction with native proteoglycans or with proteoglycans digested with either chondroitinase AC or testicular hyaluronidase. Species Crossreactivity: bovine, sheep, canine and rat