Chondroitin Sulfate Proteoglycan (NG2, CSPG)

NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.25-0.5ug/ml detects NG2 in a whole rat brain preparation. Whole rat brain preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with C5067-71. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.\nImmunohistochemistry: 1ug/ml is reported to immunostain 4% paraformaldehyde fixed adult rat brain sections.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies: \nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-14C: IgG, Fc (HRP) Pab Gt x Mo \nI1904-19C: IgG, F(ab

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SPECIFICATIONS

Catalog Number

C5067-71

Size

200ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Ughrin, Y.M., et al., J. Neurosci. 23: 175-186 (2003). 2. Levine, J.M. and A. Nishiyama, Perspec. Dev. Neurobiol. 3: 245-259 (1996). 3. Dou, C.L. and J.M. Levine, J. Neurochem. 68: 1021-1030 (1997). 4. Levine, J.M., et al., Trends in Neurosci. 24: 39-47 (2001).

Additional Info

Recognizes rat Chondroitin Sulfate Proteoglycan, Mr ~270-300kD.