CHOP (C/EBP-homologous Protein, C/EBP-homologous Protein 10, CHOP10, CHOP-10 CEBPZ, DNA Damage-inducible Transcript 3 Protein, DDIT3, DDIT-3, Growth Arrest and DNA Damage-inducible Protein GADD153, MGC4154)

CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominantnegative manner (1). CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase (2). Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death (3). Studies also found that CHOP mediates the activation of GADD34 and Ero1-La expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2a thereby stimulating protein synthesis. Ero1-La promotes oxidative stress inside the endoplasmic reticulum (ER) (4). The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4). \n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS,\n0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5064-05B

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Ron, D. and Habener, J.F. (1992) Genes Dev 6, 439-53. (2) Barone, M.V. et al. (1994) Genes Dev 8, 453-64. (3) Zinszner, H. et al. (1998) Genes Dev 12, 982-95. (4) Marciniak, S.J. et al. (2004) Genes Dev 18, 3066-77.

Additional Info

Recognizes endogenous levels of total CHOP. Species Crossreactivity: mouse, rat.