CHOP (C/EBP Homology Protein, GADD153, Growth Arrest and DNA Damage Inducible Protein 153)

CHOP (also known as GADD153, Growth Arrest and DNA Damage Inducible Protein 153, and C/EBP Homology Protein) is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP-CHOP dimer gains binding activity to other non-classical C/EBP stress related targets.\nUnder normal cellular conditions this protein is not expressed in detectable levels, but is highly upregulated during times of cellular/ER stress. Examples of CHOP inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.\n\nCellular Localization: Nuclear\n\nPositive Control: Primary human fibroblasts.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5064-05A

Size

100ul

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

1% BSA, PBS

P Type

Mab

Purity

As reported

Isotype

IgG2b

References

Wang XZ et al. Cloning of mammalian Ire1 reveals diversity in the ER stress responses. EMBO J 17:5708-17 (1998). <PUBMED:9755171>Wang XZ et al. Identification of novel stress-induced genes downstream of chop. EMBO J 17:3619-30 (1998). <PUBMED:9649432>Batchvarova N et al. Inhibition of adipogenesis by the stress-induced protein CHOP (Gadd153). EMBO J 14:4654-61 (1995). <PUBMED:7588595>

Additional Info

Species Crossreactivity: Crossreacts with Human and Mouse.

Alternative Names

C/EBP Homology Protein, GADD153, Growth Arrest and DNA Damage Inducible Protein 153