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Products ChREBP Transcription Factor Assay Kit
| Catalogue number: | 10006909 |
| Weight: | 0 |
| Form: | 96 Well |
| P type: | Assay Kits|Intracellular Signaling |
| Shipping temp: | -80 |
| Storage temp: | -80 |
| Additional info: | Cayman's ChREBP Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the ChREBP response element is immobilized onto the wells of a 96 well plate. ChREBP contained in a nuclear extract binds specifically to the ChREBP response element. ChREBP is detected by addition of specific primary antibody directed against ChREBP. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. |
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