CHRNA7, NT (CHRNA7, NACHRA7, Neuronal acetylcholine receptor subunit alpha-7) (Azide free) (HRP)

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze horseradish peroxidase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

033874-HRP

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Chernyavsky, A.I., et al. Am. J. Physiol., Cell Physiol. 299 (5), C903-C911 (2010) :\nSaccone, N.L., et al. Genes Brain Behav. 9(7):741-750(2010)\nRuano, G., et al. Pharmacogenomics 11(7):959-971(2010)\nJin, Y., et al. Int. J. Immunogenet. (2010) In press :\nSchraufstatter, I.U., et al. J Stem Cells 4(4):203-215(2009)

Additional Info

Human

Alternative Names

CHRNA7, NACHRA7, Neuronal acetylcholine receptor subunit alpha-7