The prohormone chromogranin A (CgA) is a member of the chromogranin/secretogranin protein family of regulated secretory proteins, which are widely distributed in secretory granules of endocrine, neuroendocrine, and neuronal cells. It is required for the formation of dense-core secretory granules and hormone sequestration in sympathoadrenal chromaffin cells. It functions as prohormones, giving rise to bioactive peptides, vasostatin, pancreastatin, and parastatin as a result of post-translational proteolytic processing. Peptides derived from chromogranin A have autocrine, paracrine, and endocrine activities. It also inhibit secretion of proopiomelanocortin hormone. Catestatin, another fragment of chromogranin A, inhibits the release of catecholamines from sympathoadrenal chromaffin cells by blocking the neuronal nicotinic cholinergic receptor, which is the physiologic trigger for secretion. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. It maps to 14q32 region of human chromosome.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:100
Optimal dilutions to be determined by the researcher.
Positive Control:
Pancreas
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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