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Chromogranin A (CgA, CHGA, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I)

Cat no: C5069-96T

Chromogranin A (CgA, CHGA, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I)

This monoclonal antibody is a general marker for neurohormonal peptide producing cells (APUD) and neoplasms derived from these cells. Utrastructural studies have shown that the antigen is located in the secretory granules and therefore poorly granulated endocrine cells stain weakly or not at all. This should be considered when using this antibody. \n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Acetone fixed frozen sections, formalin fixed paraffin embedded tissue): 1:10 using the Indirect method. Pretreatment is not necessary. Dilute immediately before use with PBS or Tris.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nAdrenal medulla, pancreas.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5069-96T

Size

1ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum, NHP, Prc

Form

Supplied as a liquid in TRIS/HCL, 0.09% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Lloyd, R.V., et al., 1983, Science. vol. 222, 628-630.

Additional Info

Recognizes human chromogranin A. Predominantly reacts with a 68kD protein. Species Crossreactivity: Monkey, porcine

Alternative Names

Chromogranin A (Parathyroid Secretory Protein 1), Chromogranin A Precursor

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