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ChT1 (FITC)

Cat no: C5071-50A

ChT1 (FITC)

ChT1, a member of the Ig superfamily with one V-like and one C2-like domain, is a T cell antigen that is expressed on the surface of embryonic thymocytes (day 10). In young chickens, about 90% of the thymocytes as well as a subpopulation of peripheral lymphocytes (which represent recent thymic emigrants) are ChT1 positive.1-4 Expression in the periphery declines with age and, in correlation with partial thymectomy, indicates that ChT1 can be used as an accurate marker for studying thymic function.3 This antibody can block T cell differentiation in vitro, in thymic organ cultures and in thymocyte precursors cultured on stromal cell monolayers.4\n\nApplications:\n

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SPECIFICATIONS

Catalog Number

C5071-50A

Size

500ug

Applications

FC, IP

Hosts

Mouse

Reactivities

Ch/Bird

Form

Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.

P Type

Mab

Purity

Purified

Isotype

IgG1,k

References

1. Chen, C.L., H.T.C. Chanh, and M.D. Cooper. 1984. Chicken thymocyte-specific antigen identified by monoclonal antibodies: ontogeny, tissue distribution and biochemical characterization. Eur. J. Immunol. 14:385.\n2. Houssaint, E., E. Dietz, and F.V. Jotereau. 1985. Tissue distribution and ontogenic appearance of a chicken T lymphocyte differentiation marker. Eur. J. Immunol. 15:305-308.\n3. Kong, F-K., C.L.H. Chen, and M.D. Cooper. 1998. Thymic function can be accurately monitored by the level of recent T cell emigrants in the circulation. Immunity: 8:97-104.\n4. Katevuo, K., B.A., Imhof, R. Boyd., A. Chidgey, A. Bean, D. Dunon, T.W.F. Gobel, and O. Vainio. 1999. ChT1, an Ig superfamily molecule required for T cell differentiation. J. Immunol. 162:5685-5694.

Additional Info

Recognizes the ChT1 antigen (Mr 63/104kD) on chicken thymocytes.

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