CIP2A (Cancerous Inhibitor of Protein Phosphatase 2A, p90)

Inhibition of protein phosphatase 2A (PP2A) activity has been identified as a prerequisite for the transformation of human cells. The protein, designated Cancerous Inhibitor of PP2A (CIP2A), interacts directly with the oncogenic transcription factor c-Myc, inhibits PP2A activity toward c-Myc serine 62 (S62), and thereby prevents c-Myc proteolytic degradation. In addition to its function in c-Myc stabilization, CIP2A promotes anchorage-independent cell growth and in vivo tumor formation. The oncogenic activity of CIP2A is demonstrated by transformation of human cells by overexpression of CIP2A. Importantly, CIP2A is overexpressed in two common human malignancies, head and neck squamous cell carcinoma (HNSCC) and colon cancer.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.5-2ug/ml. A band is seen at ~90kD\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control:\nH1838: HeLa Nuclear Extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C0030-03B3

Size

100ul

Applications

ELISA, ICC, IF, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b,k

References

1. Junttila, M.R., et al. CIP2A Inhibits PP2A in Human Malignancies. Cell. 130: 51-62 (2007) 2. Shi, F.D., et al. Preferential humoral immune response in prostate cancer to cellular proteins p90 and p62 in a panel of tumor associated antigens. Prostate. 63: 252-258. (2005) 3. Li W. Clin Cancer Res. Jun 15; 14(12):3722-8 (2008)

Additional Info

Recognizes a 300aa portion of human CIP2A (p90 autoantigen).