CIS1, ID (Autophagy-related Protein 31, CIK1 Suppressor Protein 1, Protein CIS1, ATG31, YDR022C, PZD196, YD9335.08c)

Suppressors of cytokine signaling (SOCS) protein family includes eight members in humans: SOCS-1 through SOCS-7 and cytokine-inducible Src-homology 2 (SH2) containing protein (CIS). All members of the family have similar structure: variable length (50-380aa) and aa sequence N-terminal region, SH2 domain in the center, and homologous aa long sequence at the C-terminus that was named SOCS box. Studies have demonstrated that animals lacking normal SOCS function exhibit high cytokine activity that leads to a variety of inflammatory and autoimmune diseases and even cancer. CIS is the first recognized and one of the well-studied members of the SOCS family. It is 258aa long with short N-terminus and shares sequence similarity with SOCS-2. CIS mRNA normally presents at very low levels in different tissues; however, its level is rapidly increased upon stimulation with IL-1, IL-4, IL-6, IL-7, IL-12, IL-13, EPO, IFNg, TNFa, prolactin, GH, and leptin. CIS inhibits signaling by binding to activated cytokine receptors; for example, CIS binds to pY401 of the EPO receptor and to pY985/pY1077 motifs of the leptin receptor. In addition, recent findings demonstrate that its C-terminal region residue Y253 is critical for CIS functioning.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5500-03B

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Bullock, A. et al. Structure 15, 1493 (2007). 2. Lavens, D. et al. Biochem. J 401, 257 (2007). 3. Lavens, D. et al. J of Cell Sc 119, 2214 (2006). 4. Cooney, R. et al. Shock 17, 83 (2002). 5. Krebs, D. et al. Stem Cells 19, 378 (2001). 6. Gregorieff, A. et al. J Biol Chem 275, 21596 (2000). 7. Hilton, D. J. et al. Biochemistry 195, 114 (1998).

Additional Info

Recognizes human CIS-1. Species Crossreactivity: rat and mouse.