CISH (CIS-1, Protein G18, Suppressor of cytokine signaling)

CISH(cytokine inducible SH2-containing protein),also called CIS, CIS-1, G18, SOCS, is an important \nnegative regulator for inflammatory signaling and belongs to the suppressors of cytokine signaling \n(SOCS) family. CIS family members are known to be cytokine-inducible negative regulators of cytokine \nsignaling. CISH controls interleukin-2 signaling, and variations of CISH with certain SNPs are \nassociated with susceptibility to bacteremia, tuberculosis and malaria. The human CISH gene is \nmapped to chromosome 3p21.3 by FISH. The mouse gene is tightly linked to the Gnai2 gene on \nchromosome 9, a region syntenic to human chromosome 3p21. CIS expression was upregulated by \nlipopolysaccharide (LPS) or Cryptosporidium parvum exposure, and this upregulation involved \ndownregulation of MIR98 and LET7, which relieved MIR98- and LET7-mediated translational repression \nof CIS. Gain- and loss-of-function studies showed that CIS accelerated degradation of IKBA and \nenhanced NFKB activation in cholangiocytes in response to LPS stimulation or C parvum exposure.\n\nUniProt Number:\nQ9NSE2\n\nGene ID:\nCISH\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144368

Size

100ug

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hu, G., Zhou, R., Liu, J., Gong, A.-Y., Eischeid, A. N., Dittman, J. W., Chen, X.-M. MicroRNA-98 and \nlet-7 confer cholangiocyte expression of cytokine-inducible Src homology 2-containing protein in \nresponse to microbial challenge. J. Immun. 183: 1617-1624, 2009.\n2. Khor, C. C., Vannberg, F. O., Chapman, S. J., Guo, H., Wong, S. H., Walley, A. J., Vukcevic, D., \nRautanen, A., Mills, T. C., Chang, K.-C., Kam, K.-M., Crampin, A. C., and 23 others. CISH and \nsusceptibility to infectious diseases. New Eng. J. Med. 362: 2092-2101, 2010.\n3. Uchida, K., Yoshimura, A., Inazawa, J., Yanagisawa, K., Osada, H., Masuda, A., Saito, T., Takahashi, \nT., Miyajima, A., Takahashi, T. Molecular cloning of CISH, chromosome assignment to 3p21.3, and \nanalysis of expression in fetal and adult tissues. Cytogenet. Cell Genet. 78: 209-212, 1997

Additional Info

Recognizes human, mouse and rat CISH. No crossreactivity with other proteins.