Citrulline

The amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation. Citrulline promotes energy and assists with the immune system. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000 detected Citrulline on 10ug of human cornified cell lysate. The human cornified cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with C5086-02. Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.\nImmunohistochemistry: 1:300 detected Citrulline in human skin tissue.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies: \nI1904-46E: IgG, H&L (HRP) Pab DkxRb\nI1904-46K: IgG, H&L, X-Adsorbed (HRP) Pab DkxRb\n\nPositive Control: \nHuman cornified cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5806-02

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Hans-Georg Kopp, Andrea T Hooper, M Johan Broekman, Scott T Avecilla, Isabelle Petit, Min Luo, Till Milde, Carlos A Ramos, Fan Zhang, Tabitha Kopp, Paul Bornstein, David K Jin, Aaron J Marcus, Shahin Rafii (2006) The Journal of clinical investigation. 116:3277-91. 2. Ishida-Yamamoto, Akemi, et al. (2002) J. Invest. Dermatol., 118: 282-7 (2002). 3. Ishida-Yamamoto, A, et al. (2000) J. Invest. Dermatol., 114: 701-5 (2000). 4. Senshu, T, et al. (1999) J. Dermatol. Sci., 21: 113-26 (1999). 5. Senshu, T, et al. (1996) Biochem. Biophys. Res. Commun., 225: 712-9 (1996).

Additional Info

Recognizes multiple deiminated keratins in human cornified skin. Thought to react preferentially with citrulline residues in glycine rich contexts. Species Sequence Homology: all species.