Cks1 (Cdc kinase subunit 1)

Cks (Cdc kinase subunit) proteins are members of the cyclin-dependent kinase (cdk) family that act as adaptors to allow the cdks to interact more efficiently with their molecular targets. Cks1 is involved in the regulation of cell cycle mitosis in normal and cancer cells. Cks1 promotes mitosis by modulating the transcriptional activation of the APC/C protein-ubiquitin ligase activator Cdc20. Cks1 has also been established as an essential cofactor in the ubiquitylation of Cdk inhibitor p27 by Skp2 ubiquitin ligase. Mammalian Cks1 binds to Skp2 and promotes the association of Skp2 with phosphorylated p27 (Thr187), which further induces ubiquitylation of p27. High expression of Cks1 may be involved in the pathogenesis of human nonsmall cell lung carcinoma.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nImmunohistochemistry: 50ug/ml\nNote: Immunohistochemistry applications with formalin-fixed, paraffin-embedded tissue sections require heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0 \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa cell lysates and human tonsil \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5818-01B

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A chromatography

Isotype

IgG1,k

Additional Info

Recognizes the 11kD human Cks1 protein. No reactivity was observed with a Cks2 recombinant protein. Cross reactivity with an unknown protein at ~35kD has been observed in Western blots of HeLa cell lysates. Reactivity has been confirmed with a Cks1 recombinant protein as well as HeLa cell lysates. IHC reactivity was confirmed with formalin-fixed, paraffin-embedded (FFPE) human tonsil sections.