Cks1, CT (Cdc kinase subunit 1)

Cks (Cdc kinase subunit ) proteins are members of the cyclin-dependent kinase (cdk) family that act as adaptors to allow the cdks to interact more efficiently with their molecular targets. Cks1 is involved in the regulation of cell cycle mitosis in normal and cancer cells. Cks1 promotes mitosis by modulating the transcriptional activation of the APC/C protein-ubiquitin ligase activator Cdc20. Cks1 has also been established as an essential cofactor in the ubiquitylation of Cdk inhibitor p27 by Skp2 ubiquitin ligase. Mammalian Cks1 binds to Skp2 and promotes the association of Skp2 with phosphorylated p27 (Thr187), which further induces ubiquitylation of p27. High expression of Cks1 may be involved in the pathogenesis of human non-small cell lung carcinoma.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nELISA (Immunogen): 0.1-1ug/ml\nWestern Blot: 1-3ug/ml\nImmunofluorescence: 10ug/ml\nImmunoprecipitation: 7ug/IP reaction\nImmunohistochemistry: 2.5ug/ml\nNote: For immunohistochemistry with formalin-fixed, paraffin-embedded tissues, Heat Induced Epotope Retrieval (HIER) with citrate buffer, pH 6.0, is recommended.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5817

Size

100ug

Applications

ELISA, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide

P Type

Pab

Purity

Purified by epitope affinity chromatography.

Isotype

IgG

References

1. Morris MC, et al. Nature 424(6943): 1009-13, 2003. 2. Ganoth D, et al. Nat Cell Biol 3(3): 321-4, 2001. 3. Inui N, et al. Biochem Biophys Res Commun 303(3):978-84, 2003.

Additional Info

Recognizes human Cks1 protein at ~11kD. Bands of unknown origin at ~20, 30 and 100kD are also observed. Reactivity: HeLa cell lysates by Western Blot, Immunofluorescence and Immunoprecipitation and with non-small cell lung carcinoma tissue by Immunohistochemistry. Western Blot with purified Cks1 and Cks2 proteins was used to confirm that this antibody does not crossreact with Cks2. Species Sequence Homology: mouse