Clathrin, Light Chain

Cellular Localization: Cell Membrane and Cytoplasmic\n\nApplications: \nSuitable for use in AP, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5835-08B

Size

100ul

Applications

ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Yeast/Fun

Form

0.01M PBS. pH 7.4

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG2b

References

Liu SH et al. A novel clathrin homolog that co-distributes with cytoskeletal components functions in the trans-Golgi network. EMBO J 20:272-84 (2001). <PUBMED:11226177>Ybe JA et al. Clathrin self-assembly is regulated by three light-chain residues controlling the formation of critical salt bridges. EMBO J 17:1297-303 (1998). <PUBMED:9482727>Liu SH et al. Regulation of clathrin assembly and trimerization defined using recombinant triskelion hubs. Cell 83:257-67 (1995). <PUBMED:7585943>Nathke IS et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68:899-910 (1992). <PUBMED:1547490>

Additional Info

Reacts specifically with the clathrin light chain. The epitope recognized by the antibody resides within amino acids 23-44 of bovine clathrin light chains, LCa and LCb. This region is not involved in heavy chain binding, and is completely conserved in all mammals. Species Crossreactivity: Crossreacts with Human, Mouse, Rat, bovine and S. cerevisiae. Not yet tested in other species.