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Claudin 1 (CLDN1)

Cat no: C5838-02C

Claudin 1 (CLDN1)

Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 2-4ug/ml for 2 hours at RT\nImmunohistochemistry (Formalin/paraffin): 1:50-1:100 for 20 minutes. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nT47D cells, Skin or Breast carcinoma.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5838-02C

Size

250ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can, NHP

Form

Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

Additional Info

Recognizes human Claudin 1 at 22kD. Species Crossreactivity: monkey, mouse, canine, and rat.

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