Claudin 11 (OSP, Oligodendrocyte Specific protein, CLDN11)

Oligodendrocyte-specific protein (OSP) is a putative four-transmembrane protein of 22kD. It is primarily expressed in oligodendrocytes of the CNS and Sertoli cells of testes in the adult mouse. OSP is the third most abundant CNS myelin protein and contributes to 7% of the total myelin protein. OSP shares sequence homology with the claudin family of tight junction (TJ) proteins. It forms TJs in cell culture and co-localizes with structures similar to TJs in myelin. Thus, it was renamed OSP/Claudin-11. OSP/Claudin-11-null mice lack intramembranous junctions in between Sertoli cells in testes and within CNS myelin sheaths, producing reproductive and neurological deficiencies. This finding indicates that OSP/Claudin-11 is necessary for the formation of TJs. OSP/Claudin-11 expression is highly regulated during development, suggesting that it may play an important role in growth and differentiation of oligodendrocytes and other cells outside the CNS. OSP/Claudin-11 forms a complex with a novel member of the tetraspanin superfamily (OAP1) and b1 integrin. This egulates proliferation and migration of oligodendrocytes, a process essential for normal myelination and repair. Claudin-11 is also expressed in the epithelial tight junctions of the choroids plexus. OSP/Claudin-11 has been implicated as an autoantigen in the development of autoimmune demyelinating disease.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence (Mouse): 2-5ug/ml\nELISA (Immunogen): 0.1-1ug/ml\nWestern Blot (Mouse): 0.5-2ug/ml. \nImmunoprecipitation (Mouse): 1-10ug/ml using NP40-mouse brain homogenates, but not for RIPA-buffer mouse brain homogenates after treating with SDS-out. The protein may have precipitated out of solution along with the SDS.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5838-62

Size

100ug

Applications

ELISA, IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Bronstein, J.M., et al., Neurology 47: 772-778 (1996). 2. Morita, K., et al., J. Cell Biol. 145: 579-588 (1999). 3. Bronstein, J.M., et al., J. Neurosci. Res. 50: 713-720 (1997). 4. Gow, A., et al., Cell 99: 649-659 (1999). 5. Bronstein, J.M., et al., J. Neurosci. Res. 60: 284-290 (2000). 6. Tiwari-Woodruff, S.K., et al., Cell Biol. 153: 295-305 (2001). 7. Wolburg, H., et al., Neurosci. Lett. 307: 77-80 (2001). 8. Bronstein, J.M., et al., J. Neurosci. Res. 59: 706-711 (2000).

Additional Info

Specific for human OSP/Claudin-11. Single band at ~22kD is observed when using C5838-62 at 0.5mg/ml or less in mouse brain homogenates. An additional band of ~44kD representing the dimerized protein may be observed at 1ug/ml.\nSpecies Crossreactivity: mouse. Species Sequence Homology: rat