Claudin 2 (CLDN2)

Tight Junctions (TJ) are specialized membrane domains that seal the intercellular spaces in epithelia and endothelia and thus contribute to the permeability barrier between lumenal and interstitial compartments. Tight junctions not only separate distinct physiological compartments, but they also confer selectivity to the transepithelial ?ux of molecules and ions through the intercellular spaces between the epithelial cells, the so-called paracellular pathway. In addition, tight junctions limit the diffusion of lipids between the apical and basolateral plasma membrane domain. Claudin and Occludin, the two types of four-transmembrane domain proteins, are the major integral membrane components of the mammalian tight junction. So far, 20 distinct claudins have been identi?ed. Mouse Claudin-2 was discovered in 1998 as a 230aa integral membrane protein localizing at tight junctions with an approximate molecular weight of 22.9kD. Claudin-1 and Claudin-2 are structurally related, and are in fact 38% identical at the amino acid sequence level. The expression of Claudin-2 is restricted to the liver and kidney, with small amounts found in the brain.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence (Rat): 1-3ug/ml\nWestern Blot (Human, mouse, rat, canine): 1-3ug/ml; Non-specific band at ~27kD observed in some lysates. In Caco-2 cells, this band appears stronger than the correct band at 22kD.\nImmunhistochemistry (FFPE): 1-2ug/ml; Confirmed with human normal colon, spleen and thyroid, colon and breast cancer tissues. Heat induced epitope retrieval with EDTA buffer, pH 8, is required prior to staining. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5838-16

Size

100ug

Applications

IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b

References

1. Anderson JM, Curr. Biol. 6:382-384 (1996). 2. Anderson JM and Van Itallie CM, Am J. Physiol., 269:G467-475 (1995). 3. Howarth AG and Stevenson BR, Adv. Struc. Biol. 4:25-39 (1996). 4. Stevenson BR and Keon BH, Annu.Rev.Cell Dev. Biol. 14:89-109 (1998). 5. Tsukita S, et al., Cell Struct. Funct. 21:381-385 (1996). 6. Yap AS et al., H, Membr. Biol. 163:159-167 (1998). 7. Saitou M, et al., J. Cell. Biol. 141:397-408 (1998). 8. Furuse M, et al., J. Cell Biol. 143:391-401 (1998). 9. Tsukita S and Furuse M, Genes Cells 3:569-573 (1998). 10. Morita K, et al., Proc Natl Acad Sci USA 96:511-516 (1999). 11. Van Itallie CM, Rahner C, Anderson JM. J Clin Invest 107(10): 1319-1327 (2001). 12. Furuse M, et al., J Cell Biol 141(7): 1539-1550, (1998). 13. Lippoldt A, et al., Neuroreport 11(7): 1427-1431 (2000).

Additional Info

Recognizes the mouse Claudin-2 protein at ~23kD. Reacts with liver and kidney homogenates. Species Crossreactivity: human (Caco-2 cell lysates), rat, canine (MDCK cell lysates)