Claudin 5 (CLDN5)

Tight junctions (TJ) are specialized regions of cell-cell adhesion in epithelial and endothelial cell sheets. Claudins are the major components tight junction strands. In the brain and lung, claudin-5 was exclusively concentrated at cell-cell borders in endothelial cells of blood vessels, but not at those of epithelial cells. In contrast, claudin-5 was restricted to endothelial cells of arteries, but not veins and capillaries in the kidney.1 It is also reported that Claudins-2,-3,-4 and -5 showed heterogenic expression and subcellular localization in the rat liver, pancreas and gut.2 Mouse anti-Claudin-5 stained 100% (20/20) of colon cancer tissues with their matched adjacent and remote mucosa. The staining patterns in both the cancer tissues or the matched mucosa are cytoplasmic and membranous. In breast carcinoma, 62% (33/53) of the tissues stained positive, but the staining pattern was mainly cytoplasmic.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemisty (FFPE): Requires Heat Induced Epitope Retrieval (HIER) using EDTA pH 8.0. Tested in human samples only. Enzyme digestion is not required.\nImmunohistochemistry: Frozen sections\nWestern Blot: 1-3ug/ml. Tested in mouse and rat samples only.\nOptimal dilutions to be determined by the researcher. \n\nPositive Control: \nColon Tissue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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