CLDN1 (Claudin-1, Senescence-associated Epithelial Membrane Protein, CLD1, SEMP1, UNQ481/PRO944)

Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions are specialized regions of cell to cell contact consisting of networking strands that act as a molecular gasket for preventing the leakage of ions, water, etc., between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. CLDN1 and CLDN2 were found to be overexpressed in colonal cancer tissues and may be useful as tumor markers and targets for the treatment of colorectal cancer. Characterization of Claudins expression in human tumors can be an additional diagnostic tool. Recent studies show that CLDN1 has gastric tumor suppressive activity and is a direct transcriptional target of RUNX3. Along with SCARB1, LDL-R, and the tetraspanin superfamily member CD81, CLDN1 has been reported to be an entry factor for the Hepatitis C virus.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunofluorescence: 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman spleen Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146448

Size

100ug

Applications

ELISA, ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Tepass U. Claudin complexities at the apical junctional complex. Nat. Cell Biol. 2003; 5:595-7. 2. Kinugasa T, Huo Q, Higashi D, et al. Selective up-regulation of claudin-1 and claudin-2 in colorectal cancer. Anticancer Res. 2007; 27:3729-34. 3. Paschoud S, Bongiovanni M, Pache JC, et al. Claudin-1 and claudin-5 expression patterns differentiate lung squamous cell carcinomas from adenocarcinomas. Mod. Pathol. 2007; 20:947-954. 4. Morohashi S, Kasumi T, Sato F, et al. Decreased expression of claudin-1 correlates with recurrence status in breast cancer. Int. J. Mol. Med. 2007; 20:139-43.

Additional Info

Recognizes human CLDN1. Species Crossreactivity: mouse and rat.