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Cleaved-PARP-1 (G215) pAb

Cat no: BS7045

Cleaved-PARP-1 (G215) pAb

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.

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SPECIFICATIONS

Catalog Number

BS7045

Size

100ug/100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 215-250 of Human PARP-1.

Species

Cleaved-PARP-1 (G215) pAb detects endogenous levels of the large fragment (89 kDa) of PARP1 resulting from caspase cleavage.

Dilutions

WB: 1:500~1:1000

Swiss Prot

P09874

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 89, 116 kDa

Alternative Names

PARP1; ADPRT; PPOL; ADPRT 1; ARTD1; Poly [ADP-ribose] polymerase 1; ADP-ribosyltransferase diphtheria toxin-like 1

SUPPLIER INFO

Bioworld Technology, Inc.

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